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Non-segmented flow injection (FI10–14) have sometimes included enzymic derivatisation in order to endow analytical Measuring intruments.1 This situation is mainly due to theĭesign of analytical pervaporators2–5 that have been used for theĭevelopment of methods based on continuous flow approachesįor a variety of analytes,2–8 which have shown the versatility ofĪutomatic methods based on either segmented (SFA9) or Process in order to accommodate the raw samples in the Analytical pervaporation is used as an advantageousĪlternative to non-chromatographic continuous separation techniques for implementing preliminary steps of the analytical Pervaporation has long been employed in industry, in competition with other traditional processes (e.g., distillation, extraction, sorption) but it has rarely been used in the analytical A fully automated approach to on-line bioprocess sample collection, dilution and monitoring of the The use of this biosensing system wasĪlso tested by the standard addition method in the fermentation product with acceptable recoveries in all instances The method shows different linear ranges depending on the situation of the catalyst and the mode used, withĮxcellent precision (RSD 3.0–5.5%) and a sampling frequency of 5 h21. Of ethanol production in an experimental laboratory built bioreactor prepared using baker’s yeast as active cells. The stopped-flow mode and AOD/POD immobilisation was selected for application to the continuous monitoring Phase or immobilised biocatalysts (in normal or stopped-flow modes in both cases). TheĮfficiency of the derivatisation reaction was tested using different flow injection approaches with either solution Peroxidase (POD) with fluorimetric detection at lex = 310 nm and lem = 415 nm of the dimer formed. Theĭerivatisation reaction was based on a two-step reaction involving alcohol oxidase (AOD) and horseradish The method combines the use of a pervaporation unit inserted in a continuous flow system with the use ofĮnzymic derivatisation and fluorimetric detection as a way of improving both selectivity and sensitivity. Received 25th June 1998, Accepted 3rd September 1998Ī new procedure for on-line monitoring of ethanol production in the wine fermentation bioprocess is presented. Luque deĭepartment of Analytical Chemistry, Faculty of Sciences, University of Córdoba, E-14004Ĭórdoba, Spain. Processes by a pervaporation–enzymic derivatisation approachį. Monitoring ethanol production during wine fermentation View Article Online / Journal Homepage / Table of Contents for this issue
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